Distribution, density and heterogeneity of canine mast cells and influence of fixation techniques |
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Authors: | P Kube Laurent Audigé Katja Küther M Welle |
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Institution: | Institute for Veterinary Pathology, Free University of Berlin, Strasse 518 Nr. 15, D-14163 Berlin, Germany, DE Institute of Virology and Immunoprophylaxis, P.O. Box, CH-3147 Mittelh?usern, Switzerland, CH Institute for Animal Pathology, University of Berne, L?nggass Strasse 122, CH-3012 Bern, Switzerland Tel. +41 31 6312 416, CH
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Abstract: | The present study was carried out to determine the physiological distribution of mast cell numbers and types in the dog according
to tissue location, staining and fixation methods. Tissue samples from stomach, duodenum, lung, lymph node, skin and uterus
were evaluated. Samples were fixed in formalin as well as in Carnoy’s fluid. The average number of mast cells was determined
using a metachromatic staining method. Protease content of mast cells was examined with a double enzyme-immunohistochemical
staining technique, using a histochemical reaction for chloroacetate esterase to detect chymase activity and an immunohistochemical
staining method for the detection of tryptase. Canine mast cells can be subdivided into formalin-sensitive and -resistant
mast cells. Three subtypes were identified according to their content of the mast cell-specific proteases tryptase (T) and
chymase (C): T-, TC- and C-mast cells. Significant differences regarding the distribution of mast cell subtypes as well as
the influence of the fixation method can be observed. This underlines the fact that data regarding mast cell heterogeneity
from other species, obtained by different fixation methods, are not comparable. This fact has to be taken into consideration
when evaluating mast cell subtypes under pathological conditions.
Accepted: 29 January 1998 |
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