| 苹果酸降解相关基因在酿酒酵母中的表达 |
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| 引用本文： | 张春晖,刘树文,夏双梅.苹果酸降解相关基因在酿酒酵母中的表达[J].中国生物工程杂志,2003,23(2):42-45. |
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| 作者姓名： | 张春晖 刘树文 夏双梅 |
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| 作者单位： | 1. 西北农林科技大学葡萄酒学院 陕西杨凌 712100;
2. 信阳农业高等专科学校植科系 河南信阳 464000 |
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| 基金项目： | 陕西省自然科学基金资助项目 |
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| 摘 要： | 微生物降酸是现代葡萄酒酿造重要工艺。将裂殖酵母苹果酸通透酶基因(mae1)和苹果酸酶基因(mae2)克隆到酿酒酵母中,构建了苹果酸酒精酵母;将mae1基因和乳酸乳球菌的苹果酸乳酸酶基因(mleS)克隆到酿酒酵母中,构建了苹果酸乳酸酵母。构建的酵母重组子能够有效地分解发酵基质中的苹果酸。
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| 关 键 词： | 苹果酸 苹果酸降解相关基因 酿酒酵母 葡萄酒 |
Cloning the Malate Degradation Related Genes in Saccharomyces cerevisiae |
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| Zhang Chunhui,Liu Shuwen,Xia Shuangmei.Cloning the Malate Degradation Related Genes in Saccharomyces cerevisiae[J].China Biotechnology,2003,23(2):42-45. |
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| Authors: | Zhang Chunhui Liu Shuwen Xia Shuangmei |
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| Institution: | Zhang Chunhui 1 Liu Shuwen 1 Xia Shuangmei 2 (1 College of Enology Northwest Sci-Tech University of Agriculture & Forestry Yangling Shanxi 712100) (2 Department of plant science Xinyang Agricultural College Xinyang Henan 464000) |
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| Abstract: | Bio-deacidificaton is a very important processing technology in modern winemaking.Due to Saccharomyces cerevisiae could not degrade malic acid functionally,with the help of molecular genetic methods,malate permease (mae1) gene and malic enzyme (mae2) gene of Schizosaccharomyces pombe could be introduced to S.cerevisiae to recombine malo-alcoholic yeast,and introduced (mae1) gene and Lactococcus lactis malolactic gene (mleS) to recombine malolactic yeast. ; |
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| Keywords: | Malate Malate degradation related genes Saccharomyces cerevisiae Wine |
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