A rapid assay for ribokinase. |
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Authors: | R K Goitein S M Parsons |
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Affiliation: | Department of Chemistry University of California Santa Barbara, California 93106 USA |
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Abstract: | A rapid method capable of detecting low levels of ribokinase is given. [γ-32P]ATP is converted to ribose 5-[32P]phosphate which is not absorbable onto charcoal. The assay is linear in enzyme concentration to a lower limit of at least 4 × 10?2 mg of enzyme/ml. |
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