Bluetongue virus-17 fusion protein ns1 expressed in Escherichia coli by pUC vectors |
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| Authors: | L F Wang R H Doi L F Chuang B I Osburn J Maisonnave E Benjamini R Y Chuang |
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| Institution: | Department of Biochemistry & Biophysics, University of California, Davis 95616. |
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| Abstract: | The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a LacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system. |
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