Proteoglycan and collagen morphology in superficially scarred rabbit cornea |
| |
Authors: | Ian M Rawe Stephen J Tuft and Keith M Meek |
| |
Institution: | (1) Oxford Research Unit, The Open University, Foxcombe Hall, BerkeleyRoad, Boars Hill, OX1 5HR Oxford, UK;(2) Moorfields Eye Hospital, City Road, EC1V 2PD London, UK |
| |
Abstract: | Summary We have examined the changes in collagen and proteoglycan morphology in superficial lamellar keratectomy wounds produced in rabbit corneas. The ultrastructural location within the tisse of keratan sulphate and chondroitin sulphate proteoglycans was demonstrated using the cationic dye Cuprolinic Blue under critical electrolyte conditions. Large proteoglycan filaments (up to 500 nm long) appeared in the early stages of wound healing; these were most common after two weeks' wound healing, after which they decreased both in number and size. At these early stages of scar formation, spaces containing proteoglycans were present amongst bundles of collagen fibrils. As proteoglycans play an important role in controlling corneal hydration, the presence of the large proteoglycan-filled spaces would result in an abnormally high water content which is found in early early scar tissue. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|