Expression of genes from the marine bacteriumAlteromonas haloplanktis 214 inEscherichia coli K-12 |
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| Authors: | MacLeod Robert A Hadley R G Szalay A A Vink Bernadette MacLeod Patricia R |
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| Institution: | (1) Department of Microbiology, Macdonald College of McGill University, H9X 1C0 Ste Anne de Bellevue, Quebec, Canada;(2) Boyce Thompson Institute for Plant Research at Cornell University, 14853 Ithaca, New York, USA;(3) Present address: Plant Genetics Inc., 1930 Fifth St., 95616 Davis, CA, USA |
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| Abstract: | DNA from the marine bacteriumAlteromonas haloplanktis 214 was partially digested withSau 3A and inserted into theBam HI site of the cloning vector pBR322. The ligation mixture was used to transformEscherichia coli HB101. The gene bank plasmid preparation obtained was used to transformEscherichia coli K-12 strain EO2717, an organism auxotrophic for histidine, arginine, adenine, uracil and thiamin. Prototrophic transformants for each of the five metabolites were isolated using appropriate minimal media for selection. Plasmids isolated from each of the transformants were shown by hybridization to containA. haloplanktis DNA and to be capable of complementing the appropriate mutation inE. coli EO2717. Restriction maps showed that each of the plasmids was different. |
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| Keywords: | Alteromonas haloplanktis Marine bacteria Escherichia coli Gene bank Gene expression Plasmids |
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