Comparison of growth properties of carrot hairy root in various bioreactors |
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Authors: | Osamu Kondo Hiroyuki Honda Masahito Taya Takeshi Kobayashi |
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Institution: | (1) Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464-01 Nagoya, Japan;(2) Present address: Department of Chemical Engineering, Faculty of Engineering Science, Osaka University, 560 Toyonaka, Osaka, Japan |
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Abstract: | Summary Growth properties of carrot hairy root cells in various bioreactors were investigated. A turbine-blade reactor and an immobilized rotating drum reactor were found to be advantageous for the hairy root culture because of a high oxygen transfer coefficient (k in L a). After 30 days of culture, 10 g/l of dry hairy root cells were obtained in both bioreactors and maximum growth rates (V
m
) were found to be 0.63 and 0.61 g/l per day for the turbine-blade reactor and immobilized rotating drum reactor, respectively. Specific growth rates () at various cultivation times were observed to be linearly proportional to X/k
l
a for both bioreactor configurations where X is the cell concentration. The estimated specific oxygen uptake rate of 0.34 mmol O2/g dry cells per hour compares fairly well with an experimental value of 0.3. |
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Keywords: | |
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