马传染性贫血病毒美洲流行株与我国弱毒疫苗株鉴别诊断方法的研究

马传染性贫血病毒(Equine infectious anemia virus,EIAV)是反转录病毒科慢病毒属的成员,是马传染性贫血病的病原。二十世纪七十年代我国就研制出马传染性贫血驴白细胞弱毒疫苗,成为世界第一个成功地应用该疫苗控制了我国的马传贫的发生[1]。而且我国的马传贫弱毒疫苗对异源的美国、古巴和阿根廷等毒株也有很高的保护率[2]。因此将我国的马传贫驴细胞弱毒疫苗推向国际市场成为可能。然而目前制约该苗出口的技术问题是现行的OIE推荐的琼脂扩散实验和ELISA等血清学方法不能鉴别自然感染马与我国弱毒疫苗免疫马,针对这个关键问题,本试验…

The Study on the Differentiation between the EIAV American prevalent strain and Attenuated Vaccine strain

In order to develop an assay to distinguish the infection of Equine infectious anemia virus(EIAV)American strains from Donkey-leukocyte attenuated virus(DLV)strain,eight primers were designed based on the comparison of complete sequences of four EIAV American strains and DLV.Corresponding viral genome fragments were amplified and analyzed from donkey leucocytes by PCR using these eight primers.Four primers from gag gene were selected to detect the differences between EIAV American strains and DLV.Six horses were inoculated with DLV and two with EIAV American strains,the Wyoming strain and the Argentina strain,respectively.Two fragments,675bp and 400bp,were amplified from DLV-infected cells,while only one segment of 675bp was obtained from cells inoculated with American strains.Our results indicate that this nest multiplex PCR can be used to distinguish EIAV American strains from the donkey leucocyte-attenuated vaccine strain of EIAV.