植物金属硫蛋白Ⅱ型与谷胱甘肽在烟草中的表达和合成特性

利用从印度芥菜(Brassica juncea)中获得的金属硫蛋白2型（BjMT2）基因通过农杆菌介导法转入模式植物种烟草中。转基因烟草在经过继代组织培养和抗生素筛选以及PCR和Western blot检测后，确定目的基因BjMT-2被整合到烟草基因组中并具备表达功能。与对照野生型相比，转入金属硫蛋白2型的烟草在植物形态和根部发育方面都发生了显著的性状变化。在正常条件下，转基因植物叶片组织中谷胱甘肽的含量与野生型没有明显差别，但经过100 mmol·L^-1 Cu²⁺胁迫后，谷胱甘肽的含量明显高于野生型。在转基因植物细胞膜系统中V-型ATP酶的活性低于野生型，而P-型ATP酶的活性则表现出明显的升高趋势。

BjMT2,a Metallothionein Type2 from Brassica juncea, Expression and GSH Synthesis in Tobacco Plant

Plant Metallothionein type2 isolated from Brassica juncea was transformed into tobacco leaves via agrobacterium tumefaciens-mediated transformation. The transformation was confirmed by PCR and Western blot analysis. The transgenic plants differentiated in their growing phenotypes and in root elongation compared with those of wild types. The level of GSH in transformed and wild type of tobacco leaves did not show significant variation but increased extremely in the transformed leaves after 100 mmol·L^-1 Cu²⁺ stress. The analysis of ATP hydrolysis showed that the P-ATPase activities were higher in the microsomal fraction prepared from transformed tobacco leaves than those from wild types. However, the V-ATPase activities were declined in comparison with the wild types.