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Solid phase synthesis of growth hormone-release inhibiting factor
Authors:D H Coy  E J Coy  A Arimura  A V Schally
Institution:Veterans Administration Hospital and Tulane University School of Medicine New Orleans, Louisiana 70146 USA
Abstract:The growth hormone-release inhibiting factor (GH-RIF) tetradecapeptide
/></figure>, was prepared by the cyclization of a linear, disulfhydryl peptide intermediate by treatment with potassium ferricyanide. The linear peptide was synthesized by an automated solid-phase technique. The cyclic material inhibited the secretion of radioimmunoassayable growth hormone <math><mtext>in vitro</mtext></math> at doses as low as 0.1 μg. A high molecular weight compound formed during the cyclization reaction and believed to be predominantly dimer also possessed considerable inhibitory activity.</td>
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