Avocado shoot culture, plantlet development and net CO2 assimilation in an ambient and CO2 enhanced environment |
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Authors: | Witjaksono Bruce A Schaffer Angel M Colls Richard E Litz Pamela A Moon |
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Institution: | (1) Tropical Research and Education Center, University of Florida, 18905 SW 280 Street, 33031-3314 Homestead, Florida |
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Abstract: | Summary The proliferation and survival of avocado nodal cultures of juvenile origin were affected by the form and concentration of
nitrogen. Optimum growth was achieved on modified Murashige and Skoog medium containing 67% KNO3 and 33% NH4NO3 with total N of 40 mM supplemented with 100 mg l−1
myo-inositol, 1 mg l−1 thiamine HCl, 30 g l−1 sucrose, and 4.44 μM BA with a 16-h photoperiod (120–150 μmol m−2 s−1). Proliferating shoots and plantlets were photosynthetically active. Better shoot growth and accumulation of higher biomass
occurred in a CO2-enriched environment than under ambient CO2 conditions. CO2 assimilation efficiency, however, was higher under the latter conditions than in a CO2-enhanced environment, e.g., 31±7 and 17±2 μmol CO2 m−2 s−1, respectively. The net CO2 assimilation rates of in vitro grown plantlets were comparable to those of seedlings ex vitro. |
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Keywords: | Persea mericana Mill avocado micropropagation CO2 nitrogen |
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