Effects of temperature and water activity on <Emphasis Type="Italic">Lecanicillium</Emphasis> spp. conidia germination and growth,and mycosis of <Emphasis Type="Italic">Pissodes strobi</Emphasis> |
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Authors: | Email author" target="_blank">Harry?H?KopeEmail author René?I?Alfaro Robert?Lavallée |
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Institution: | (1) Pacific Forestry Centre, Canadian Forest Service, Victoria, BC, Canada, V8Z 1M5;(2) Centre de foresterie des Laurentides, Service canadien des forêts, Sainte-Foy, QC, Canada, G1V 4C7;(3) Present address: Forest Practices Branch, British Columbia Ministry of Forests and Range, 727 Fisgard Street, Victoria, BC, Canada, V8W 1R8 |
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Abstract: | Selecting entomopathogenic fungal isolates for use as biocontrol agents requires an assessment of their growth and virulence
characteristics as affected by environmental conditions. Here we demonstrate a wide temperature and moisture range for colony
growth, effective conidial germination and virulence against Pissodes
strobi Peck (white pine weevil) of several isolates of Lecanicillium Gams and Zare, an entomopathogenic fungus distributed worldwide and indigenous to forests on Vancouver Island, British Columbia,
Canada. In order to examine the potential Lecanicillium as a biological control agent, the pathogenicity of isolates collected from different geographical locations on P. strobi cadavers was assessed, and colony growth at different temperatures was evaluated. Colony growth was evident between 5 and
30°C, with optimal growth occurring at 25°C. Various combinations of water activity (0.55, 0.76, 0.85 and 0.99 a
w) and temperature (10, 15, 20, and 25°C) were also used to evaluate environmental impacts on conidial germination and cumulative
mycosis of adult P. strobi. Certain Lecanicillium isolates displayed xerophilic (0.85 a
w) or psychrophilic (10°C) growth optima. Ultimately, identifying the abiotic limits of this entomopathogenic fungus will be used to determine which isolates have
potential for future in situ biocontrol trials. |
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Keywords: | Biological control Entomopathogenic fungi White pine weevil Abiotic tolerance Pissodes strobi Lecanicillium |
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