趋磁螺菌遗传操作体系的建立及磁小体缺失突变株的筛选

由于MagnetospirillumgryphiswaldenseMSR 1缺少简便有效的遗传操作体系和对常见抗生素的抗性 ,致使对该菌磁小体生物合成的机制等研究工作进展缓慢。为此建立了一套比较简便有效的遗传操作体系 ,其中包括 :以平板封膜培养技术获得单菌落、在选择性培养液中进行接合转移遗传因子 ,以液体培养和磁铁吸附技术筛选突变子。利用此体系 ,通过接合转座诱变技术 ,获得了 2个磁小体缺失突变株 ,为研究该菌磁小体合成的分子遗传学提供了技术支撑

Development of A Genetic Manipulation System and Screening of Magnetosome Deleted Mutants for Magnetospirillum gryphiswaldense

Progress in the genetic analysis of the mechanism of magnetosome biosynthesis in Magnetospirillum gryphiswaldense MSR 1 has been hampered by the lack of an appropriate genetic manipulation system and its antibiotic resistant mutants. Here reported on the establishment of a genetic manipulation system that can be used more readily than that of the others for Magnetospirillum. This system included method of solid medium plate enveloped with Parafilm to form colony, conjugational gene transfer in a selective medium, screening the nonmagnetic mutants by magnet adsorption technique. Two magnetosome deleted mutants were constructed by conjugative transposon mutagensis and the application of this genetic system. This system and the two nonmagnetic mutants will be very useful in study of the molecular mechanism of magnetosome biosynthesis in magnetotactic bacteria.