Methylation and expression of bovine leukemia proviral DNA |
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| Authors: | S V Kashmiri R Mehdi P Gupta J F Ferrer |
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| Affiliation: | 1. State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China;2. CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China;3. Center for Molecular Medicine, Children’s Hospital, Fudan University and Shanghai Key Laboratory of Medical Epigenetics, International Laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China;4. School of Life Science and Technology, ShanghaiTech University, Shanghai, China;5. School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China;1. The Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK;2. Theriogenology department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt;3. Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK;4. Centre for Mechanochemical Cell Biology & Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Gibbet Hill, Coventry CV4 7AL, UK;5. University Hospitals Coventry and Warwickshire NHS Trust, Coventry CV2 2DX, UK;6. Medical Research Council Centre for Reproductive Health, University of Edinburgh, Edinburgh EH16 4TJ, UK;1. College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, China;2. Medical College, Nankai University, Tianjin, 300500, China;1. Laboratory for Cell Function Dynamics, BSI, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan;2. Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan;3. King’s College London Centre for Stem Cells and Regenerative Medicine, 28th Floor, Tower Wing, Guy’s Campus, Great Maze Pond, London SE1 9RT, UK;4. Biotechnological Optics Research Team, Center for Advanced Photonics, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan;5. Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Koutou 135-0064, Japan;6. Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan;1. The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, 127 Dongming Road, Zhengzhou, 450008, China;2. The Fifth Affiliated Hospital of Zhengzhou University, No. 3 Kangfu Front Street, Zhengzhou, 450052, China |
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| Abstract: | In vivo, the BLV proviral DNA usually resides in a transcriptionally inert state, and is hypermethylated. Upon short-term in vitro cultivation of the neoplastic or non-neoplastic lymphoid cells, the viral genome becomes transcriptionally active but without detectable change in its methylation state. Proviral DNA was found to be methylated in one but not in the other long-term BLV producer cell line examined. These data indicate that hypermethylation of proviral DNA may not be responsible for the covert nature of BLV infection in vivo. |
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