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Isolation and characterization of nuclear microsatellite loci in the tropical tree Corythophora alta (Lecythidaceae)
Authors:M. B. Hamilton
Abstract:Studies of tropical plant population structure and gene flow are needed to estimate genetic repercussions of tropical deforestation. Twelve microsatellite loci were isolated for Corythophora alta (Lecythidaceae), a canopy tree at the Biological Dynamics of Forest Fragments Project, near Manaus, Brazil. These microsatellites will be used to estimate population structure and infer seed paternity. Loci were isolated with a subtractive hybridization method. Loci averaged 10.7 alleles per locus with high levels of heterozygosity. Four loci had significant deficits and one locus had a significant excess of heterozygotes compared to Hardy–Weinberg expectations. Many loci had high average probabilities of paternity exclusion.
Keywords:Corythorphora alta  forest fragmentation  Lecythidaceae  microsatellite  seed paternity  tropical tree
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