脑缺血再灌流大鼠海马hsp70及bcl—2基因的表达

为进一步了解中枢神经系统中选择性易损伤的分子机制，采用大鼠短暂前脑缺血再灌流损伤动物模型，应用Ｎｏｒｔｈｅｒｎ杂交、原位杂交及免疫组织化学方法，检测了ｈｓｐ７０及ｂｃｌ－２基因的表达及其组织学分布。发现易损伤的海马ＣＡ１区锥体细胞出现ｈｓｐ７０基因的诱导表达，ＢＣＬ－２蛋白合成受抑制；而耐受缺血的海马ＣＡ３区锥体细胞则明显地持续表达ＢＣＬ－２蛋白，却未见明显的ｈｓｐ７０基因表达。因此提示，ｈｓｐ７０基因的表达是神经元缺血的应激指征，也可能对神经元有保护作用；ＢＣＬ－２蛋白对神经元有保护作用

THE EXPRESSION OF hsp70 AND BCL 2 GENES IN HIPPOCAMPUS OF THE RATS EXPOSED TO CEREBRAL ISCHEMIA AND REPERFUSION

In order to study the molecular mechanism of the selective vulnerability in central nervous system,the expression and distribution of hsp70 and BCL 2 gene were detected by using Northern blot analysis , in situ hybridization and histochemistry method in transient forebrain ischemia and reperfusion rats It was found that hsp70 gene expression occurred and the synthesis of BCL 2 protein was inhibited in hippocampalCA 1 region vulnerable to ischemia,while BCL 2 protein was stained strongly and the signals of hsp70 were not observed in CA 3 region resistant to ischemia The results indicate that the expression of hsp70 gene may be not only as a marker for neuron ischemia,but also play a protective role in the neuronal injury BCL 2, meanwhile, may have neuro protective effect on the ischemic neurons