离体灌流大鼠左右心肌的应激蛋白增加的非一致性

在病理状况及不同生理条件刺激时，左右心功能和结构改变的不一致已被广泛发现，但是对于造成左右心不一致变化的原因尚缺乏认识。本研究利用双向电泳方法分析和比较了应激蛋白在常氧及低氧灌流的离体大鼠左右心肌的诱导。结果表明，三个分子量为７０ｋＤａ（Ｓｐ６８，７０和７２）ｐＩ６．３－７．３的应激蛋白在左右心肌中均显著增加，并且应激蛋白在右心的合成量要高于左心。这说明右心对缺血灌流刺激的应答不同于左心，应激蛋白对左右心的保护程度不一样。此外，左右心的过氧化氢酶活力均显著下降，提示对离体灌流的大鼠心脏，氧化应激可能是诱导应激蛋白合成的一个重要原因，并且左右心应激蛋白增加不一致的原因很可能与左右心自身的结构及所处的状况有关。

Discordance of increase in stress proteins in right and left myocardium of perfused isolated rat heart]

Discordance of functional and structural changes in right and left myocardium has been considerably discovered in various physiologically stimulated hearts and patients with cardiopathy, but the reasons for that have not yet been known. In the present study, induction of stress proteins, i.e. heat shock proteins, was analyzed and compared between the left and right myocardium of normoxic and hypoxic perfused isolated rat hearts by the methods of two dimensional electrophoresis and silver staining. The results showed that three Hsp70 isoforms (molecular weight 68, 70 and 72 k mu) with pI ranging from 6.3 to 7.3 were increased distinctly in both right and left myocardium under the conditions of perfusion as mentioned above. Moreover, the amount of the stress proteins increased in the right myocardiums was higher than that in the left myocardium, indicating that the response of right heart to the stimulus of hypoxic or normoxic perfusions is different from the reaction of left heart, and the extent of protection in left and right heart by stress proteins is unlike. In addition, the activity of catalase was found to be obviously declined in all perfused isolated hearts. It was suggested that the different increase in stress proteins may be due to different structure and status of right and left ventricle and the oxidative stress may be one of the important reasons to induce or enhance the synthesis of stress proteins.