SARS-CoV推测N蛋白功能结构的生物信息学研究

目的:利用生物信息学方法理论分析不同地区来源的SARS冠状病毒(SARSCoV)推断N蛋白的基因组与氨基酸序列的差异及分子生物学特征以及基因突变对蛋白结构功能的影响。方法:针对GenBank上发布的来自不同国家地区的15条SARSCoV基因组序列,采用生物信息学软件分析其推测N蛋白的CDS和氨基酸序列,分别找出突变位点并预测其等电点及功能结构域。结果:SARSCoV推测N蛋白基因组序列存在5个变异位点导致蛋白序列有4个位点发生突变。在该蛋白上发现四个有意义的低成分复杂性区域;未发现卷曲螺旋、跨膜螺旋和信号肽序列。基因突变造成4条序列在功能位点数量上减少,但未影响抗原决定簇。预测发现两个保守的Domain和一个丝氨酸富集区。结论:不同地区来源的15条推测N蛋白序列的变异很少。基因突变导致部分序列功能位点数量发生改变,但未影响抗原决定簇的数量。

Bioinformatic Analysis of Fanction and Structure of Putative Nucleocapsid Protein Sequences of SARS-CoV

Objective:The objective of the paper was to analyze the characteristics of putative nucleocapsid protein sequence of Severe Acute Respiratory Syndrome Coronavirus(SARS CoV)and provide information for the further study of their structure and function.Methods:A series of bioinformatic tools and software such as Clustal X,DNATools,DNASIS,SMART,TMHMM Server,ProtParam Tools and so on were used to compare and analyze the SARS CoV nucleocapsid protein CDS as well as coding protein sequences from GenBank.Results:The gene sequence of 15 different sourced SARS CoV putative nucleocapsid proteins has 5 variances resulting in 4 mutations occurred in its amino acid sequence.Four significant low compositional complexities were predicted and no coiled coil,TMH and signal peptide were found.Conclusion:The gene mutation resulted in the functional sites changed in some sequences and did not influence the antigenic determinants.