中国35个苦荞审定品种EST-SSR指纹图谱构建与遗传多样性分析

核心引物对种质资源遗传多样性分析、品种鉴定、指纹图谱构建等研究具有重要价值。本研究以35个苦荞（Fagopyrum tataricum（L.） Gaertn）审定品种为材料,从91对苦荞EST-SSR引物中筛选出50对多态性引物。综合考虑引物多态性信息量（PIC）大小、鉴别力（DP）,筛选出等位变异位点数在2~4,PIC值在0.60~0.78之间的6对引物（SSR9007、SSR6873、SSR7642、SSR2234、SSR6789、SSR68216）构建了供试品种的分子指纹图谱。遗传多样性聚类分析结果表明,供试品种的相似系数为0.50~0.99。当遗传相似系数为0.60时,可将供试品种分为4大类群,其中54.3%的供试品种被聚为一类,表明苦荞审定品种遗传组成差异较小,遗传基础狭窄。聚类结果表明各类群间没有明显的地域分布趋势,但能较好的反映供试品种间的亲缘关系。

Construction of EST-SSR fingerprinting and analysis of genetic diversity of thirty-five registered tartary buckwheat cultivars (Fagopyrum tataricum) in China

Core primers are important in genetic diversity analysis, cultivar identification, and fingerprinting construction. In this study, 91 pairs of EST-SSR primers were screened using 35 registered tartary buckwheat cultivars, with 50 primers showing polymorphism. Based on the polymorphism information content (PIC) and discrimination power (DP) results, a core set of six SSR primers (SSR9007, SSR6873, SSR7642, SSR2234, SSR6789, and SSR68216) were further selected to construct fingerprinting of the 35 cultivars, with alleles amplified by each pair of primers ranging from 2 to 4 and PIC values ranging from 0.60 to 0.78. The UPGMA cluster analysis showed that the genetic similarity of the 35 cultivars varied from 0.50 to 0.99. The cultivars were divided into four groups based on a genetic similarity of 0.60, with 54.3% remaining clustered together, indicating a close genetic relationship and narrow genetic basis. The genetic relationship was in accordance with their breeding history, but was not affected by their geographic differences.