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Comparison of Phenacoccus solenopsis specimens from different regions of Pakistan using COI molecular barcoding (Hemiptera: Pseudococcidae) |
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| Authors: | Hayat Badshah Farman Ullah Hayat Zada Qamar Zeb Ruidar A Shah Paul-André Calatayud |
| Institution: | 1. Entomology Section, Agricultural Research Institute Tarnab, Peshawar 24330, Pakistan;2. Department of Plant Protection, The University of Agriculture, Peshawar, Pakistan;3. UMR EGCE (Evolution, Génome, Comportement, Ecologie), CNRS-IRD-Univ. Paris-Sud, IDEEV, Université Paris-Saclay, Gif-sur-Yvette Cedex, France;4. Institut de Recherche pour le Développement (IRD) c/o International Centre of Insect Physiology and Ecology (icipe), P.O. Box 30772-00100, Nairobi, Kenya |
| Abstract: | Because correct identification of insects is crucial for pest management involving chemical or biological control agents, we have used a molecular approach to identify and characterize specimens of the cotton pest Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae) present in different regions of Pakistan. The specimens were analyzed through DNA sequence analysis of their mitochondrial COI (mtCOI) gene using an improved procedure that could distinguish between the pest and its associated parasitoid. Our analysis showed no variation among the mealybug specimens from different geographical locations of Pakistan and confirmed that this is the same species and haplotype that is infesting cotton plants in other parts of Asia. This information will assist in the development of biological control programs against P. solenopsis in Pakistan and other Asian countries. |
| Keywords: | cotton pest pest management mealybug Pseudococcidae biological control |