Peptides in the hemolymph of Hermetia illucens larvae completely inhibit the growth of Klebsiella pneumonia in vitro and in vivo |
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| Institution: | 1. Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Republic of Korea;2. Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, Republic of Korea;3. Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea;1. Ojeong Eco-Resilience Institute, Korea University, Seoul 02841, Republic of Korea;2. Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea;3. Institute of Environment and Ecology, OJERI, Korea University, Seoul 02841, Republic of Korea;1. Integrated Pest Management Laboratory, Department of Entomology, University of Agriculture, Faisalabad, Pakistan;2. Department of Entomology, University of Agriculture, Faisalabad, Depalpur Campus, Okara, Pakistan;3. Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, PR China;4. Institute of Geography, Fujian Normal University, 350007, China;5. College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, PR China;6. Insect Biodiversity and Conservation Group, Department of Entomology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan;7. Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia;8. Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia;9. Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia;10. Department of Botany, Government College University Faisalabad, Pakistan;1. School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China;2. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou, China;1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing 210014, China;2. College of Life Sciences, Huaibei Normal University, Huaibei 235000, China;3. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;4. Institute of Plant Protection, Jiangxi Academy of Agricultural Science, Nanchang 330200, China;1. Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;2. Southern Insect Management Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, MS 38776, USA;3. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | Extensive use of antibiotics has caused the microbial resistance to rise drastically within the last few decades, and new approaches are therefore needed to develop effective antibacterial substances. In this study, we identified peptide in the hemolymph of Hermetia illucens larvae using reverse-phase chromatography, HPLC and Nano-LC-ESI-MS/MS system. We investigated the antibacterial effect of HP/F9 peptides against Klebsiella pneumoniae in vitro and in vivo. The peptide effectively inhibited the growth of K. pneumoniae in vitro and completely removed K. pneumoniae from the lungs of mice. Importantly, peptides (22,000 Da, HP/F9) successfully reduced lung inflammation upon K. pneumoniae infection. These results indicate that the HP/F9 peptide from H. illucens larva can effectively protect the mouse from K. pneumoniae infection. HP/F9 could be a new candidate for the development of effective antibacterial substance. |
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| Keywords: | Antimicrobial peptide High performance liquid chromatography (HPLC) Antibiotics Probiotics |
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