Potentially Pathogenic Acanthamoeba Isolated from a Hospital in Brazil |
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Authors: | Ana Maris Carlesso Geórgia Lazzari Artuso Karin Caumo Marilise Brittes Rott |
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Institution: | 1. Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, CEP 90050170, Brazil 2. PPG Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, CEP 90050170, Brazil 3. Department of Microbiology, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, CEP 90050170, Brazil
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Abstract: | Studies on free-living amoebae (FLA), has been increased in recent years, especially related to the genus Acanthamoeba, because these organisms are widely found in the environment. The present work isolated and characterized this organism from
biofilms and dust in hospital environment. 135 samples were collected in 15 different environments in a hospital at the south
of Brazil. Thirty-one (23%) isolates were identified as morphologically belonging to the Acanthamoeba genus and 10 of these were submitted to temperature and osmotolerance tests as criterion for evaluation of the viability
and pathogenicity. The tests indicate that four (40%) of these isolates could be potentially pathogenic because grew at high
temperature (40°C) and osmolarity (mannitol 1 M). Some isolates genotypes were determined after ribosomal DNA sequencing.
These data revealed that three dust isolates belong to T4, two biofilm isolates to T5 and one dust isolate to T3 genotype.
Therefore, Acanthamoeba found in the hospital environment represents a risk for people that circulate there. |
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