Restricted diffusion of integral membrane proteins and polyphosphoinositides leads to their depletion in microvesicles released from human erythrocytes. |
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| Authors: | C Hagelberg and D Allan |
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| Affiliation: | Inositol Lipid Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709. |
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| Abstract: | Radioactive PtdIns(3)P was detected in human platelets incubated with [32P]Pi, but remained unaffected by thrombin treatment. In contrast, [32P]PtdIns(3,4)P2 was absent from resting platelets, but was produced by thrombin-activated platelets in a dose- and time-dependent manner. [32P]PtdInsP3 was never found under these conditions. These changes are similar to those elicited in other cells by platelet-derived growth factor or the oncogene product pp60c-src. |
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