大鼠黄体细胞酪氨酸含量及hCG,cAMP和孕酮对含量的影响

经PMSG-hCG处理的未成年雌性大鼠卵巢,用胶原酶-DNA酶溶液消化,制成黄体细胞悬浮液。预培育1h后,加入不同浓度的hCG、cAMP及孕酮;并在加入hCG(10mIU/ml)、cAMP(2.5mmol/L)或孕酮(1nmol/L)的同时分别加入苯丙氨酸或放线菌酮,再培育1.5h,取细胞悬浮液400μl,用薄层层析扫描技术测其酪氨酸含量。结果:黄体细胞内有一定量内源酪氨酸,hCG,cAMP和孕酮均可明显促进酪氨酸的释放(P<0.05).苯丙氨酸对酪氨酸的含量无影响。酪氨酸释放也不依赖蛋白质合成过程。

THE CONTENT OF TYROSINE IN RAT LUTEAL CELL AND THE CHANGE CAUSED BY hCG,cAMP AND PROGESTERONE

The ovaries taken from the immature female rats primed with PMSG-hCG were digested with collagenase-DNAase solution to obtain the luteal cell suspensions. Luteal cells were then incubated with different doses of hCG, cAMP or progesterone for 1.5 hours. The contents of tyrosine in luteal cell suspensions were determined by thin layer chromatogram using dual-wavelength chromatogram scanner. It was found that the content of tyrosine in rat luteal cell suspensions was 1.41 + 0.24 nmol/L/10(6) cells after one hour incubation. hCG, cAMP and progesterone all could significantly increase the content of tyrosine in the suspensions, suggesting the release of "endogenous tyrosine". The increase of tyrosine was not due to increased transformation of phenylalanine, the precursor of tyrosine, and increased protein synthesis, because both phenylalanine and cycloheximide failed to influence such increase.