Micropropagation of Spilanthes acmella Murr. |
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Authors: | KV Saritha E Prakash N Ramamurthy CV Naidu |
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Institution: | (1) Department of Biotechnology, S.V. University, Tirupati, 517502, A.P., India;(2) Biotechnology Research Centre, Tirupati, 517507, A.P., India |
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Abstract: | Multiple shoots of Spilanthes acmella Murr. were induced from hypocotyl segments obtained from 1-week-old seedlings on Murashige and Skoog's (MS) medium containing benzyladenine (BA), isopentenyl adenine, and naphthaleneacetic acid (NAA). High frequency shoot proliferation (95 %) and maximum number of shoots per explant (10 ± 0.6) were recorded with 0.5 mg dm–3 BA in combination with 0.1 mg dm–3 NAA. A proliferation was achieved by repeatedly subculturing the nodal segments on shoot multiplication medium. About 95 % of the in vitro shoots developed roots after transfer to half strength MS medium containing indole-3-butyric acid (1.0 mg dm–3). 95 % of the plantlets were successfully acclimatized and established in soil. Transplanted plantlets showed normal flowering without any morphological variation. |
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Keywords: | direct regeneration growth regulators medicinal plant tissue culture |
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