Isolation and characterization of polymorphic microsatellite markers in the red snow crab (Chionoecetes japonicus) |
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Authors: | Azuma N Abe S |
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Institution: | Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate-shi, Hokkaido 041-8611, Japan. |
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Abstract: | A total of 12 polymorphic microsatellite DNA loci were isolated from the red snow crab, Chionoecetes japonicus (Brachyura: Majidae), one of important fisheries resources in the Far East. The number of alleles observed at each locus ranged from two to 19, with the observed and expected heterozygosities of 0.125-0.875 and 0.156-0.949, respectively, suggesting these loci to be a useful molecular marker for population analysis in this species. Of the 12 loci, seven also were available for genotyping of the snow crab, Chionoecetes opilio, implying these loci as a useful molecular marker in the genus Chionoecetes. |
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Keywords: | biotinylated oligo probes Chionoecetes magnetic beads microsatellites red snow crab snow crab |
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