Selection and biochemical studies of pyrimidine-requiring mutants of Lactobacillus plantarum |
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| Authors: | A Masson B Kammerer J-C Hubert |
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| Institution: | Laboratoire de Microbiologie et de Génétique de l'UniversitéLouis-Pasteur, Unitéde Recherche Associée au CNRS no. D1481, 'Dynamique, Évolution et Expression de Génomes de Microorganismes', Strasbourg, France |
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| Abstract: | Mutagenesis and mutant enrichment in Lactobacillus plantarum, a lactic acid bacterium used in silage, sauerkraut and sausage fabrication, were studied. In optimal conditions, auxotrophic mutants were obtained that permitted investigation of the de novo pyrimidine biosynthesis. Uracil-requiring mutants were characterized for their enzymatic defect, in aspartate transcarbamylase (ATCase), dihydro-orotase (DHOase), dihydro-orotate dehydrogenase (DHOdehase), orotidine monophosphate pyrophosphorylase (OMPppase) or in orotidine monophosphate decarboxylase (OMPdecase). The five enzymatic activities are totally repressed by uracil in the growth medium. |
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