Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing |
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| Authors: | Andreotti Renato Pérez de León Adalberto A Dowd Scot E Guerrero Felix D Bendele Kylie G Scoles Glen A |
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| Institution: | (1) EMBRAPA Beef Cattle, BR 262 km. 04, Caixa postal n. 154, 79.002-970 Campo Grande, MS, Brazil;(2) USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Rd., 78028 Kerrville, TX, USA;(3) Research and Testing Laboratory, Pathogenius, and Spirostat Technologies, 4321 Marsha Sharp Fwy., 79407 Lubbock, TX, USA;(4) USDA-ARS Animal Disease Research Unit, Washington State University, 3003 ADBF, 99164 Pullman, WA, USA |
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| Abstract: | Background Ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The cattle tick,
Rhipicephalus (Boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. Tick microbiomes remain
largely unexplored. The objective of this study was to explore the R. microplus microbiome by applying the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to characterize
its bacterial diversity. Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult
females derived from samples of R. microplus collected during outbreaks in southern Texas. |
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