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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease |
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| Authors: | Scott J Gratz Alexander M Cummings Jennifer N Nguyen Danielle C Hamm Laura K Donohue Melissa M Harrison Jill Wildonger Kate M O’Connor-Giles |
| Abstract: | We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila. |
| Keywords: | CRISPR RNA Cas9 homologous recombination genome engineering Drosophila |