10个品系小鼠的RAPD 分析

小鼠是应用最为广泛的实验动物,预先了解品系或近交系的遗传纯度或系间遗传距离,对于试验材料的选取和实验结果分析都有很大帮助.传统的实验动物遗传监测多限于基因表型的检测,检测位点在整个基因组中所占比例小,分布不均.由于RAPD方法的优势,用一系列引物可检测出覆盖整个基因组的遗传改变,在动、植物品系鉴定和遗传监测方面已取得大量成果(陈洪等,1994;沈曦等,1999),但对多品系小鼠的研究报道较少.因此,本研究选用10个常用小鼠品系,筛选较好的多态标记,建立各品系的RAPD图谱,以区分不同品系的小鼠,为今后的质量监测工作提供参照标准;同时根据RAPD扩增结果计算出各品系间的相对遗传距离,在DNA水平探讨不同品系小鼠间的亲缘关系.

RAPD ANALYSIS OF TEN STRAINS OF MICE

Genetic variation and relationship in ten common strains of mice were studied with RAPD. We attempted to identify DNA polymorphisms that distinguish the ten strains of mice using 80 random primers,21 of the 80 primers generated polymorphic profile among the mice. Each of the strains could easily be distinguished from the others using these polymorphic primers. The results indicated that (1)among the ten strains, the higher genetic distances were detected between DBA/2 and T739,615,C57BL/6J, the genetic distances were 0 162,0 166,and 0 168 respectively; (2) KM had a closer relationship with TA2,BALB/c,BALB/c nu, the genetic distances were 0 060,0 066 and 0 065 respectively; (3)BALB/c and BALB/c nu had the closest relationship. These results coincided with the history of origins and breeds. suitable for mouse genetic monitoring and strains identification.