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Tetrahymena Thermophila: Growth In Synthetic Nutrient Medium In the Presence and Absence of Glucose |
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| Authors: | L SZABLEWSKI P HOVE ANDREASEN A TIEDTKE J FLORIN-CHRISTENSEN M FLORIN-CHRISTENSEN L RASMUSSEN |
| Institution: | Department of General Biology and Parasitology, Institute of Biostructure, Medical Academy, 02-004 Warsaw, Poland;Institute of Molecular Biology, Odense University, 5230 Odense, Denmark;Institute of Zoology. 5 Schlossplatz, 4400 Münster, Federal Republic of Germany;Center for Parasite and Vector Research, National University, 1900 La Plata, Argentina;Institute of Medical Biology, Odense University, 5230 Odense, Denmark |
| Abstract: | ABSTRACT This report contains the newest directions for preparation of the synthetic nutrient medium for some Tetrahymena species that do not require lipids. In the standard medium T. thermophila. strains SB 210 and 281, multiply at 37°C with doubling times of around 2 h and at 26°C around 5 h. We have established multiplication rates as functions of variations in the composition of the medium. In media in which all components are present at one-third of the normal concentrations and only the essential amino acids are included, growth and multiplication become sharply dependent on glucose in strain SB 281. Such media may be used for selection and enrichment of certain specified cell lines. |
| Keywords: | Absence of glucose growth rates synthetic nutrient medium |