Continuous-flow enrichment of a strain ofErwinia carotovora having specificity for highly methylated pectin |
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| Authors: | M Leticia Almengor-Hecht Alan T Bull |
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| Institution: | (1) 30 calle 15-31, Zona 12, Guatemala City, C.A.;(2) Department of Applied Biology, University of Wales Institute of Science and Technology, CF1 3NU Cardiff, Wales, UK |
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| Abstract: | A pectinolytic bacterium was isolated from a mixed microbial population by means of a chemostat enrichment procedure. The bacterium, which was identified asErwinia carotovora, grew only on highly methylated pectin and produced a pectin lysase which released unsaturated monomer and dimer from 71% esterified citrus pectin. The pectin lyase was inducible only by pectins having a high methyl content and in pectin-limited chemostats its synthesis passed through a maximum at a dilution rate close to 0.04h-1. |
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| Keywords: | Chemostat enrichment Erwinia carotovora Pectin lyase |
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