大鼠脑内远位触液神经元p38丝裂原活化蛋白激酶的分布及在噪声应激时的表达

目的：观察脑内远位触液神经元内p-p38丝裂原活化蛋白激酶（MAPK）的分布及其在噪声应激时的表达。方法：用霍乱毒素亚单位B与辣根过氧化物酶复合物（CB-HRP）标记和免疫组织化学相结合的双重标记技术．观察SD大鼠脑实质内远位触液神经元中p-p38MAPK的分布：进一步制作噪声应激动物模型,观察噪声应激后该类神经元中p-p38MAPK的表达变化。结果：在脑干的特定部位恒定出现被CB-HRP标记的两组神经细胞簇,其他脑区未见CB-HRP标记神经细胞簇。不予应激刺激,该细胞簇内仅有个别神经元见有CB-HRP／p—p38MAPK;噪声应激刺激1d时,上述特定部位细胞簇的CB-HRP／p-p38MAPK双重标记神经元数目没有明显变化;噪音应激刺激5d时,CB-HRP／p—p38MAPK双重标记神经元数目较对照组显著增多（P〈0．05）;噪音应激刺激10d时CB-HRP／p—p38MAPK双重标记神经元数目较对照组显著增多（P〈0．05）;噪音应激刺激20d时,CB-HRP／p—p38MAPK双重标记神经元数目较对照组显著增多（P〈0．01）：结论：在脑干特定部位恒定存在的两组被CBHRP标记的细胞团为远位触液神经元,其中少数触液神经元有p-p38MAPK表达,且当给予动物噪声应激刺激时,p-p38MAPK免疫阳性神经元和CB-HRP／p—p38MAPK双重标记神经元数量显著增加,提示脑实质内的这种远位触液神经元中的P—p38MAPK可能参与了机体对噪声应激的信息传递或调控,其作用随应激天数增加而日趋增强．

Distribution and expression of p38 MAPK in the distal cerebrospinal fluid contacting neurons in brain of rat by noise stress

Aim:To observe the distribution and expression of p-p38MAPK in the distal cerebrospinal fluid contacting neurons in brain of rat by noise stress.Methods:By a doubl-labelled method combing the tracing of CB-HRP and the immuhistochemical technique p-p38MAPK,the distribution and expression of p-p38MAPK in the distal cerebrospinal fluid contacting neurons(csf-cn) were observed following noise stress.Expression of p-p38MAPK and double-labelled of CB-HRP/p-p38MAPK were also observed in rat brain after noise stress.Results:Two groups of CB-HRP labeled neuron clusters consistently appeared in certain regions of the brainstem but none in other regions of the brain.Without noise stress exposure,only a few neurons were found double-labeled by CB-HRP/p-p38MAPK.After 1 day noise stress exposure,only few neurons double-labeled by CB-HRP/p-p38MAPK were observed in the above-mentioned regions.After 5 days,the number of neurons double-labeled by CB-HRP/p-p38MAPK increased significantly compared with the control group(P<0.05).After 10 days,the number of neurons double-labeled by CB-HRP/p-p38MAPK increased significantly compared with the control group(P<0.05).After 20 days,both of the numbers of neurons double-labeled by CB-HRP/p-p38MAPK increased significantly compared with that of the control group(P<0.01).Conclution:Two groups of distal cerebrospinal fluid contacting neuron clusters consistently existed in certain regions of the brain parenchyma,and in these clusters only a few neurons contained p-p38MAPK.After noise stress exposure of different durations(days 1,5,10,20),the number of distal cerebrospinal fluid contacting neurons with p-p38MAPK increased significantly with increasing days.The results indicate that distal cerebrospinal fluid contacting neurons are special neurons existing consistently in brain,including distal cerebrospinal fluid contacting neurons with p-p38MAPK which may participate in the whole procedure of signal transduction or central modulation in noise stress response and play greater roles with increasing days.