Preparation and conformational characterization of Cr(III) hemoglobin |
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Authors: | MD Fiechtner G McLendon MW Bailey |
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Institution: | Department of Chemistry University of Rochester Rochester, New York 14627 USA |
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Abstract: | Chromium(III) substituted hemoglobin has been prepared. Circular dichroism spectra in the UV region have been recorded in the presence and absence of the allosteric affector inositol hexaphosphate. The reactivity with bromthymol blue and p-mercuribenzoate has been measured. All data indicate a T state (or T state-like) structure, whereas an R structure would be expected from the chromium stereochemistry. Similarities to cobalt(III) hemoglobin suggest that the chromium derivative also exists as an internal hemichrome. Thus, despite major tertiary structure differences, “denatured” hemichromes may have a quaternary structure quite similar to deoxyhemoglobin. |
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Keywords: | Hb hemoglobin CrPPIX chromium(III)-protoporphyrin IX DMF N N-dimethylformamide IHP inositol hexaphosphate BTB bromthymol blue PIPES piperazine-N N′-bis[2-ethane sulfonic acid] |
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