A simple direct spectrophotometric assay for 24,25-dihydroxyvitamin D3 |
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| Authors: | Barbara E. Dreyer David B.P. Goodman |
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| Affiliation: | 1. Department of Internal Medicine, Yale University Medical School, New Haven, Connecticut 06510, USA;2. Department of Pathology, and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 USA |
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| Abstract: | A simple reproducible assay to determine the concentration of 24,25-dihydroxyvitamin D3 in blood plasma is described. This technique employs quantitation of the steroid by direct spectrophotometric analysis, rather than competitive protein binding assay after partition and high-pressure liquid chromatographic purification of plasma extracts. The concentration of metabolite observed in normal adult plasma is 2.4 ± 1.1 ng/ml. |
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| Keywords: | All correspondence and reprint requests should be addressed to: Dr. David B. P. Goodman Box 709 Hospital of the University of Pennsylvania 3400 Spruce Street Philadelphia Pa. 19104. |
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