Structural analysis of Tpn1, a transposable element isolated from Japanese morning glory bearing variegated flowers |
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Authors: | Atsushi Hoshino Yoshishige Inagaki and Shigeru Iida |
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Institution: | (1) Department of Biological Science and Technology, Science University of Tokyo, 278 Yamazaki, Noda-shi, Chiba, Japan;(2) Department of Health Science, Faculty of Pharmaceutical Sciences, University of Tokyo, 113 Hongo, Bunkyo-ku, Tokyo, Japan |
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Abstract: | The 6.4 kb transposable element Tpn1 belonging to the En/Spm family was found within one of the DFR (dihydroflavonol-4-reductase) genes for anthocyanin biosynthesis in a line of Japanese morning glory (Pharbitis nil) bearing variegated flowers. Sequencing of the Tpn1 element revealed that it is 6412 by long and carries 28-bp perfect terminal inverted repeats. Its subterminal repetitive regions, believed to be the cis-acting sequences for transposition, show striking structural features. Twenty-two copies of the 10-bp sequence motif GACAACGGTT can be found as direct or inverted repeats within 650 by of the 5 end of the element, and 33 copies of the sequence motif lie within 800 by of the 3 terminus. All these 22 copies of the sequence motif near the 5 terminus and 30 copies in the 3 terminal region are arranged as inverted repeats and 3–8 by AT-rich sequences are detected between these inverted repeats. In addition, four copies of 122-bp tandem repeats and six copies of 104-bp tandem repeats are present in the 5 and 3 subterminal repetitive regions, respectively. No large open reading frame characteristic of autonomous elements of the En/Spm family can be detected within the element. The results are discussed with respect to heritable changes in flower variegation in this line of Japanese morning glory. |
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Keywords: | Transposable element Tpn1 structure Japanese morning glory En/Spm family Subterminal repetitive region |
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