Chemical composition of lipopolysaccharides from Legionella bozemanii and Legionella longbeachae |
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Authors: | Anders Sonesson Erik Jantzen Torill Tangen Ulrich Zähringer |
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Institution: | (1) National Institute of Public Health, Geitmyrsveien 75, N-0462 Oslo, Norway;(2) Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Parkallee 22, D-23845 Borstel, Germany;(3) Present address: Astra Draco AB, P.O. Box 34, S-22100 Lund, Sweden |
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Abstract: | Lipopolysaccharides (LPS) from Legionella bozemanii serogroup 1 and Legionella longbeachae serogroup 1 were subjected to chemical analyses. The lipid A part of both LPSs contained 2,3-dideoxy-2,3-diamino-d-glucose as major constituents and d-glucosamine and glycerol as minor constituents of the sugar backbone structure. Both LPSs exhibited a very complex fatty acid composition. Twenty amide-linked 3-hydroxy fatty acids were detected in LPS of L. longbeachae, whereas seventeen were encountered in LPS of L. bozemanii. Both LPSs contained nine ester-linked nonhydroxy fatty acids and the unique long-chain fatty acids 27-oxo-octacosanoic acid, 29-oxotriacontanoic acid, heptacosane-1,27-dioic acid and nonacosane-1,29-dioic acid. SDS-PAGE showed that L. bozemanii produced smooth-form LPS, whereas L. longbeachae LPS was mainly of the R-type. Composition analyses were in accordance with these electrophoretic patterns. d-Quinovosamine and l-fucosamine constituted 80 mol% of the polysaccharide part of L. bozemanii LPS. Other sugars identified were d-glucosamine, d-mannose, d-glucose, l-rhamnose, d-glycero-d-manno-heptose, l-glycero-d-mannoheptose, 2-keto-3-deoxy-octonic acid and glycerol. The polysaccharide chain from LPS of L. longbeachae appeared to be shorter, but composed of the same sugars except l-fucosamine. Both LPSs contained glycerol phosphate and glucosamine phosphate and L. longbeachae LPS contained in addition glucose phosphate.Abbreviations
EI
Electron impact
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GlcN3N
2,3-Diamino-2,3-dideoxy-d-glucose
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HPAEC
High pH anion-exchange chromatography
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Kdo
2-Keto-3-deoxy-octonic acid
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LPS
Lipopolysaccharide
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PCP
Phenol/chloroform/petroleum ether solvent
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PED
Pulsed electrochemical detection
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PS
Polysaccharide
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TFA
Trifluoroacetyl
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TMS
Trimethylsilyl |
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Keywords: | Taxonomy Chemical analyses Long-chain fatty acids 2 3-Diamino-2 d-glucose" target="_blank">3-dideoxy-d-glucose |
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