The primary structure of the operons coding for Shigella dysenteriae toxin and temperature phage H30 shiga-like toxin |
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| Authors: | Kabishev" target="_blank">A A Kozlov YuVKabishev E V Lukyanov A A Bayev |
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| Institution: | Institute of Molecular Biology, USSR Academy of Sciences, Moscow. |
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| Abstract: | Nucleotide(nt) sequences were determined for the toxin (SHT) operon present in the chromosome of Shigella dysenteriae 1 and for the shiga-like toxin (SLT) operon found in the lambdoid phage H30 genome. The coding sequences of the sht and slt genes differ in 4 nt with 1 nt change responsible for an amino acid replacement. The deduced amino acid sequence in the A chain of the toxins is highly homologous to that of the A chain of ricin, a plant toxin. SHT-coding mRNAs were detected by mapping the 5' termini and using blot-hybridisation; one of them was more abundant and coded only for the B subunit of SHT while the other (bi-cistronic mRNA) encoded both subunits. An IS element related to the IS3 element of Escherichia coli was found in the chromosome of S. dysenteriae near the sht operon. |
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