The serotyping of Azospirillum spp. by cell-gold immunoblotting |
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| Authors: | Vladimir A. Bogatyrev Lev A. Dykman Larisa Yu. Matora Boris I. Schwartsburd |
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| Affiliation: | Institute of Biochemistry and Physiology of Plants and Microorganisms, Academy of Sciences of Russia, Saratov. |
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| Abstract: | Ten Azospirillum strains were serotyped using the method of cell-gold immunoblotting (dot-blot immune-overlay assay). Colloidal gold-protein A conjugate was used. Antibodies raised against the whole cells showed strain specificity and interacted mainly with carbohydrate antigens on the cell surface. Immunological identity for A. brasilense Sp 245 and Sp 107 strains was found. Cell-gold immunoblotting can be recommended for serotyping of a wide variety of bacterial strains. |
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| Keywords: | Azospirillum Serotyping Immunoblotting Colloidal gold |
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