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| 青海油菜蜂花粉黄酮类化合物含量的研究 | |
| 引用本文： | 魏永生 郑敏燕 王永宁 石玉平 卢挺. 青海油菜蜂花粉黄酮类化合物含量的研究[J]. 西北植物学报, 2004, 24(2): 301-305 |
| 作者姓名： | 魏永生 郑敏燕 王永宁 石玉平 卢挺 |
| 作者单位： | 1. 青海师范大学,化学系,西宁,810008 2. 青海省产品质量监督检验所,西宁,810008 |
| 基金项目： | 科技部农业科技成果转化资金资助项目(2002630080415)，青海省科技厅资助项目(2002-N-119) |
| 摘 要： | 采用正交试验设计，研究了青海油菜蜂花粉中黄酮类化合物的最佳提取条件，分别采用HPLC法和比色法测定了黄酮类化合物的含量。最佳提取条件是选用95％的乙醇作为溶剂，以10倍总量的乙醇用量，水浴热回流提取。以槲皮素、山奈酚、异鼠李素为对照品，采用HPLC法，测定出黄酮类化合物平均含量为3．194％；以芦丁为对照物，采用比色法，测定出总黄酮平均含量为3．70％。 |
| 关 键 词： | 油菜 蜂花粉 黄酮 HPLC 比色法 |
| 文章编号： | 1000-4025(2004)02-0301-05 |
A study on the content of flavonoids in bee-pollen of Brassica campestris from Qinghai |
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| WEI Yong-sheng,ZHENG Min-yan,WANG Yong-ning,SHI Yu-ping,LU Ting. A study on the content of flavonoids in bee-pollen of Brassica campestris from Qinghai[J]. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(2): 301-305 | |
| Authors: | WEI Yong-sheng ZHENG Min-yan WANG Yong-ning SHI Yu-ping LU Ting |
| Affiliation: | WEI Yong-sheng~1,ZHENG Min-yan~1,WANG Yong-ning~1,SHI Yu-ping~1,LU Ting~2 |
| Abstract: | The optimum condition of extracting the flavonoids in bee-pollen of Brassica campestris Qinghai was studied by the orthogonal experiment design. The content of flavonoids in bee-pollen was determined by HPLC and colorimetry. The optimum conditions were as follows: the addition of solvent (95% ethanol) was ten times the addition of solute, then reflux in heated water-bath. The average content of flavonoids was 3.194% by HPLC, 3.70% by colorimetry with quercetin, kaempferol , isorhamnetin and rutin as standard samples. |
| Keywords: | Brassica campestris bee-pollen flavonoids HPLC colorimetry |
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