| 一株丙型肝炎病毒缺陷株NS5A基因片段的序列分析 |
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| 引用本文： | 朱分禄,逯好英.一株丙型肝炎病毒缺陷株NS5A基因片段的序列分析[J].Virologica Sinica,1998,13(2):182-184. |
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| 作者姓名： | 朱分禄 逯好英 |
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| 作者单位： | 第二军医大学微生物学教研室，军事医学科学院基础医学研究所，北京佑安医院 |
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| 摘 要： | Ｑ丙型肝炎是由丙型肝炎病毒（ＨＣＶ）引起的一种严重的传染病。丙型肝炎病毒主要通过输血和应用不洁的血液制品传播，所以利用敏感、特异的检测方法筛选献血员对丙型肝炎的预防尤为重要。现在第二代ＨＣＶ检测试剂已大批应用，加入ＮＳ５Ａ抗原的第三代试剂也正在研制中...
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| 关 键 词： | 丙型肝炎病毒，缺陷株，序列分析 |
Sequencing of a cDNA Fragment of NS5A Region of the Defective Hepatitis C Virus |
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| Zhu Fenlu\ Lu Haoying\ Meng Qinghua,et al.Sequencing of a cDNA Fragment of NS5A Region of the Defective Hepatitis C Virus[J].中国病毒学(英文版),1998,13(2):182-184. |
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| Authors: | Zhu Fenlu\ Lu Haoying\ Meng Qinghua |
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| Abstract: | Sensitive and specific anti HCV diagnostic kits are very important for preventing of the transmission of hepatitis C virus. Adding HCV NS5A antigen in diagnostic kits may increase the sensitivity of diagnostic reagents. The result of sequencing of NS5A cDNA fragment showed that it shares over 90% nucleotide acid and amino acid homology with HCV BK etc., so the isolate of this study belongs to HCV II. Besides, a mutation at 411nt in NS5A segment, which created a stop codon TGA in the reading frame was found, indicating that the isolate might be a defective virus. Perhaps the defective virus causes the persistent infection of HCV. |
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| Keywords: | Hepatitis C virus Defective virus Sequence analysis |
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