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Release of nucleotide-cleaving acid phosphatase from carrot cells grown in suspension culture |
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| Authors: | G. Ciarrocchi R. Cella E. Nielsen |
| Affiliation: | Istituto di Genetica Biochimica ed Evoluzionistica del CNR, via S. Epifanio, 14, 1-27100 Pavia;E. Nielsen, Istituto di Microbiologia e Fisiologia Vegetale dell'Università, 1-27100 Pavia, Italy.;Istituto di Microbiologia e Fisiologia Vegetale dell'Università, 1-27100 Pavia, Italy. |
| Abstract: | Carrot ( Daucus carota L. cv. Lunga di Amsterdam) cells grown in suspension culture release into the culture medium a phosphatase capable of converting deoxyribo- as well as ribonucleoside triphosphates into nucleosides. The enzyme activity requires acidic pH and allows a prompt utilization of phosphorylated DNA precursors as measured by tritiated dTTP incorporation. Such utilization is partially inhibited by inorganic phosphate and completely inhibited by ATP. |
| Keywords: | Thymidine thymidine triphosphate acid phosphatase inhibition |