Production of recombinant allergens in plants |
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Authors: | Georg Schmidt Gabriele Gadermaier Heidi Pertl Marc Siegert Kirsi-Marja Oksman-Caldentey Anneli Ritala Martin Himly Gerhard Obermeyer Fatima Ferreira |
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Institution: | (1) Christian Doppler Laboratory for Allergy Diagnosis and Therapy, Department of Molecular Biology, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria;(2) Molecular Plant Biophysics and Biotechnology, Department of Molecular Biology, University of Salzburg, Salzburg, Austria;(3) VTT Technical Research Centre of Finland, Espoo, Finland |
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Abstract: | A large percentage of allergenic proteins are of plant origin. Hence, plant-based expression systems are considered ideal
for the recombinant production of certain allergens. First attempts to establish production of plant-derived allergens in
plants focused on transient expression in Nicotiana benthamiana infected with recombinant viral vectors. Accordingly, allergens from birch and mugwort pollen, as well as from apple have
been expressed in plants. Production of house dust mite allergens has been achieved by Agrobacterium-mediated transformation of tobacco plants. Beside the use of plants as production systems, other approaches have focused
on the development of edible vaccines expressing allergens or epitopes thereof, which bypasses the need of allergen purification.
The potential of this approach has been convincingly demonstrated for transgenic rice seeds expressing seven dominant human
T cell epitopes derived from Japanese cedar pollen allergens. Parallel to efforts in developing recombinant-based diagnostic
and therapeutic reagents, different gene-silencing approaches have been used to decrease the expression of allergenic proteins
in allergen sources. In this way hypoallergenic ryegrass, soybean, rice, apple, and tomato were developed. |
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Keywords: | Allergy Expression system Green biotechnology Molecular farming Recombinant protein |
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