湖北光敏核不育水稻农垦58S叶片60 kD蛋白特异性及其功能的讨论

湖北光敏核不育水稻(Hubei photoperiod-sensitive genic male-sterile rice, HPGMR)是在我国发现并开始应用研究的。人们在应用研究中发现的许多问题,如光敏不育特性转育到籼稻上多变成温敏不育等,很难达成共识。加强机理研究已成为光敏水稻研究深入发展的当务之急。除了对不同时期“农垦58”与58S的代谢做各种分析外,寻找特异蛋白与特异基因是光敏水稻机理研究中两个重要方面。在蛋白研究方面,目前已报道发现35kD、45kD、60kD、61kD等与光敏不育材料有关的特异蛋白。这些结果表明,在“农垦58”与58S之间是可以找到特异蛋白的,但还不能确定这些蛋白与光敏不育性状的关系。为了解决已发现的特异蛋白与光敏不育性状的相关性问题,我们采用多对照的方法,对已知在农垦58S中稳定表达而在“农垦58”中不出现的60kD蛋白做了进一步的研究。

DISCUSSION ON THE FUNCTION AND SPECIFICITY OF THE 60 kD LEAF PROTEIN IN HUBEI PHOTOPERIOD-SENSITIVE GENIC MALE-STERILE RICE NONGKEN 58S

The specificity of the 60 kD leaf protein previously reported in Hubei photoperiod-sensitive genie male-sterile rice (HPGMR) Nongken 58S (Oryza saliva L.) was reexamined. The leaf proteins from 28 rice strains were tested with two-dimentional electrophoresis. It was found that although this protein was stably tested in Nongken 58S, not in Nongken 58, it was also expressed in many other ssp. japonica rice strains. The possible functions of this protein and the importance of setting proper controls in the study of such complicated system were discussed.