Mutagenicity of methylated N-nitrosopiperidines in Saccharomyces cerevisiae. |
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| Authors: | F W Larimer D W Ramey W Lijinsky J L Epler |
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| Institution: | 1. Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tenn. 37830 U.S.A.;1. Frederick Cancer Research Center, Post Office Box B, Frederick, Md. 21701 U.S.A. |
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| Abstract: | N-Nitrosopiperidine (NP) and a number of methylated derivatives were examined for mutagenicity in Saccharomyces cerevisiae. NP, 2-methyl-NP, 3-methyl-NP, 4-methyl-NP and 3,5-dimethyl-NP were mutagens when metabolic activation (rat-liver microsomes) was provided. 2,6-Dimethyl-NP was not a mutagen. The NPs giving a positive response stimulated forward mutation to canavanine resistance (CAN1 leads to can1) and reversion of the his1-7 missense marker. Neither locus revertants nor suppressors of the lys1-1 ochre marker were induced, nor were revertants of the putative frameshift hom3-10. |
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| Keywords: | ARG L-arginine CAN L-canavanine HIS L-histidine LYS L-lysine MFD mutation frequency decline NP N-nitrosopiperidine SC synthetic complete THR L-threonine YEPD yeast extract-peptone-dextrose |
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