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Genotypic variation in anther culture and effect of ovary coculture in durum wheat |
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| Authors: | J'Aiti F. Benlhabib O. Sharma H. C. El Jaafari S. El Hadrami I. |
| Affiliation: | (1) Laboratory of Plant Physiology, Department of Biology, University Cadi Ayyad, Faculty of Sciences Semlalia, B.P. 2390, 40001 Marrakech, Morocco;(2) Laboratory of Tissues Culture and Cytogenetic, Department of Agronomy, Rabat-Institutes, IAV Hassan II, B.P. 6202, 10101 Rabat, Morocco;(3) Department of Agronomy, Purdue University, West Lafayette, IN, USA;(4) Laboratoire de Biotechnologie et Amélioration des Plantes, Faculté des Sciences, 50000 Meknes, Maroc |
| Abstract: | The response of anthers to in vitro culture and the effect of coculture of ovaries on anther culturability have been studied in responsive and recalcitrant cultivars of durum wheat (Triticum turgidum ssp. durum) from Morocco and ICARDA. A large genotypic-dependence of anther culture has been shown in 18 cultivars. Their response in term of callus and embryo induction varied from 0 to 13%. Coculture of ovaries with anthers enhanced the response of the most responsive genotype (cv. Sarif) and removed the recalcitrance in Cocorit and Isly cultivars. However, there was no effect of anther-ovary coculture on green plant regeneration. The implication of the genome and the media conditioning by the ovaries on anther response is discussed. This revised version was published online in June 2006 with corrections to the Cover Date. |
| Keywords: | androgenic potential anther culture anther-ovary coculture durum wheat |
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