Transformation of <Emphasis Type="Italic">Lyophyllum decastes</Emphasis> by particle bombardment |
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| Authors: | Masahide Sunagawa Hitoshi Murata Yasumasa Miyazaki Masaya Nakamura |
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| Institution: | (1) Department of Applied Microbiology, Forestry and Forest Products Research Institute, Ibaraki 305-8687, Japan |
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| Abstract: | The basidiomycete Lyophyllum decastes was transformed by means of particle bombardment. We isolated five transformants under twelve conditions differing in the
two parameters of target distance and helium pressure. The transformation frequency was one transformant/μg DNA. In the transformants,
plasmid DNAs were integrated into the genomic DNA and stably maintained. This is the first report on transformation of L. decastes by particle bombardment. |
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| Keywords: | Lyophyllum decastes Particle bombardment Transformation |
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