Use of a surface plasmon resonance biosensor for the determination of binding constants of transiently expressed recombinant antibody to its antigen |
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| Authors: | Kondo Masahide Taya Toshiki Matsuba Takao Fushimi Noriko Inouye Kuniyo Kidokoro Shun-ichi Yasukawa Kiyoshi |
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| Institution: | (1) Tokyo Research Center, Tosoh Corporation, 2743-1 Hayakawa, Ayase-shi, 252 Kanagawa, Japan;(2) Sagami Chemical Research Center, 4-4-1 Nishionuma, Sagamihara, 229 Kanagawa, Japan;(3) Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Oiwakecho, Kitashirakawa, Sakyo-ku, 606-01 Kyoto, Japan |
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| Abstract: | Summary By using a commercially available surface plasmon resonance (SPR) biosensor, the values of the association rate constant (kass), dissociation rate constant (kdiss), and association constant (KA = kass / kdiss) for binding to the antigens were determined. They were almost the same for the recombinant antibody expressed in COS cells, CHO cells, and mouse hybridoma cells. The system of transient expression of the recombinant antibody (Ab) in COS cells and SPR analysis of the supernatant should be useful for rapid expression and evaluation of the binding ability of large numbers of engineered Abs. |
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