Effect of clofibrate on the adenosine triphosphatase activity of rat liver mitochondria. |
| |
Authors: | S R Panini C K Ramakrishna Kurup |
| |
Institution: | Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India |
| |
Abstract: | The antihypercholesterolemic drug clofibrate (ethyl-α-p-chlorophenoxyisobutyrate) stimulated the latent ATPase activity and “superstimulated” the uncoupler-induced ATPase activity of rat-liver mitochondria. Addition of clofibrate decreased the turbidity of mitochondrial suspensions and released considerable amount of mitochondrial protein into solution. In these properties it closely resembled detergents like Triton X-100 and deoxycholate. However, unlike the detergents, clofibrate required the presence of a permeant cation for its disruptive action. Also, it was without any such effect on sonic submitochondrial particles. The drug enhanced the uptake of both Mg2 and Cl? by mitochondria suggesting that osmotic swelling precedes lysis. Sonic submitochondrial particles prepared in the presence of clofibrate showed a greater yield and comparable ATPase activity. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|