Nickel resistance of Alcaligenes denitrificans strain 4a-2 is chromosomally coded |
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Authors: | Parjit Kaur Katrin Roß Roman A Siddiqui Hans G Schlegel |
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Institution: | (1) Institut für Mikrobiologie, Grisebachstrasse 8, D-3400 Göttingen, Federal Republic of Germany |
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Abstract: | A newly isolated aerobic hydrogen-oxidizing bacterium, Alcaligenes denitrificans strain 4a-2, differs from related autotrophic bacteria by containing only a single cytoplasmic, NAD-reducing hydrogenase, and by its high resistance to nickel ions, i.e. tolerance to 20 mM NiCl2. Strain 4a-2 harbors a single plasmid of about 250 kb. On helper-assisted mating of 4a-2 with Alcaligenes eutrophus strains H16,G29, and M85 nickelresistant transconjugants were selected; these did not contain the donor plasmid, however. All three transconjugants tolerated 3 to 10 mM NiCl2. The resistance was constitutively expressed. DNA/DNA hybridization showed homology with EcoRI-digested DNA of the wild type 4a-2 and transconjugants using a DNA probe containing nickel resistance genes of pMOL28. This indicated that the 4a-2 nickel resistance genes are located on the chromosome. |
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Keywords: | Alcaligenes denitrificans Alcaligenes eutrophus Nickel resistance Hydrogenase |
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